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rnase  (Thermo Fisher)


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  • 99

    Structured Review

    Thermo Fisher rnase
    Rnase, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 99/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/rnase/Water%2C+RNase%2C+DNase-free/pmc13234486-106-8-13
    Average 99 stars, based on 1 article reviews
    rnase - by Bioz Stars, 2026-11
    99/100 stars

    Images

    Related Articles

    Membrane:

    Article Title: Bacillus subtilis pb2441 partially attenuates hepatic steatosis with transcriptomic changes broadly similar to dapagliflozin in a Western diet mouse model.
    Article Snippet: .. The spin column was then placed in a new 1.5 mL collection tube, and RNA was eluted by adding 50 μL of RNase-free water directly to the membrane and centrifuging for 1 minute at 8,000 × g. RNA concentration and purity were assessed using 1 μL of total RNA on a NanoDrop 8000 spectrophotometer (Thermo Fisher Scientific, Waltham, MA, USA). .. RNA integrity was evaluated using a Bioanalyzer 2100 (Agilent Technologies, Santa Clara, CA, USA), and RNA quality was reported as the RNA Integrity Number (RIN).

    Concentration Assay:

    Article Title: Bacillus subtilis pb2441 partially attenuates hepatic steatosis with transcriptomic changes broadly similar to dapagliflozin in a Western diet mouse model.
    Article Snippet: .. The spin column was then placed in a new 1.5 mL collection tube, and RNA was eluted by adding 50 μL of RNase-free water directly to the membrane and centrifuging for 1 minute at 8,000 × g. RNA concentration and purity were assessed using 1 μL of total RNA on a NanoDrop 8000 spectrophotometer (Thermo Fisher Scientific, Waltham, MA, USA). .. RNA integrity was evaluated using a Bioanalyzer 2100 (Agilent Technologies, Santa Clara, CA, USA), and RNA quality was reported as the RNA Integrity Number (RIN).

    Article Title: Phenotypic and genotypic assessment of antimicrobial and biocide resistance in enterobacteria from anaerobic digestion of dairy waste: implications for environmental and public health
    Article Snippet: Genomic DNA was then extracted from the resulting bacterial cultures using a commercial kit, following the manufacturer’s instructions (PureLink Genomic DNA Mini Kit, Invitrogen). .. The DNA was quantified using a Nanodrop spectrophotometer (Thermo Scientific) and diluted to a concentration of 10 ng/μL in ultrapure, RNase- and DNase-free water (Invitrogen). ..

    Article Title: IGFBP1: A Key Regulatory Gene in the Oncogenesis and Progression of Esophageal Cancer
    Article Snippet: .. Total RNA was resuspended in RNase-free water, and RNA purity and concentration were determined using a NanoDrop spectrophotometer(Thermo Fisher Scientific, Wilmington, NC, USA). .. One microgram (1 μg) of total RNA was reverse transcribed into complementary DNA (cDNA) with the HiScript III RT SuperMix for qPCR (+gDNA wiper) kit (Vazyme Biotech Co., Ltd., Nanjing, China).

    Spectrophotometry:

    Article Title: Bacillus subtilis pb2441 partially attenuates hepatic steatosis with transcriptomic changes broadly similar to dapagliflozin in a Western diet mouse model.
    Article Snippet: .. The spin column was then placed in a new 1.5 mL collection tube, and RNA was eluted by adding 50 μL of RNase-free water directly to the membrane and centrifuging for 1 minute at 8,000 × g. RNA concentration and purity were assessed using 1 μL of total RNA on a NanoDrop 8000 spectrophotometer (Thermo Fisher Scientific, Waltham, MA, USA). .. RNA integrity was evaluated using a Bioanalyzer 2100 (Agilent Technologies, Santa Clara, CA, USA), and RNA quality was reported as the RNA Integrity Number (RIN).

    Article Title: Phenotypic and genotypic assessment of antimicrobial and biocide resistance in enterobacteria from anaerobic digestion of dairy waste: implications for environmental and public health
    Article Snippet: Genomic DNA was then extracted from the resulting bacterial cultures using a commercial kit, following the manufacturer’s instructions (PureLink Genomic DNA Mini Kit, Invitrogen). .. The DNA was quantified using a Nanodrop spectrophotometer (Thermo Scientific) and diluted to a concentration of 10 ng/μL in ultrapure, RNase- and DNase-free water (Invitrogen). ..

    Article Title: Profiling heterologous 3′ terminator-dependent expression variation in Aspergillus oryzae
    Article Snippet: .. After sequential washing steps, RNA was eluted in RNase-free water, quantified using a NanoDrop spectrophotometer, assessed for purity, and stored at −80 °C until use. .. cDNA synthesis was performed using 100 ng of total RNA and the iScript cDNA Synthesis Kit (Bio-Rad) according to the manufacturer's instructions.

    Reverse Transcription Polymerase Chain Reaction:

    Article Title: Alkylating agents activate an SLFN11-dependent vulnerability that confers PARP-1 inhibitor sensitivity in kidney cancer.
    Article Snippet: Immunofluorescent analysis was carried out with a Zeiss Observer Z1 fluorescence microscope. .. Quantitative reverse transcription-polymerase chain reaction (qRT-PCR) assay Total RNA was isolated using TRIzol reagent (ThermoFisher) and cleaned with RNase-free DNase I (ThermoFisher). cDNA was synthesized using the iScript cDNA synthesis kit (Bio-Rad). .. Realtime PCR was performed by a CFX-96 real-time system (Bio-Rad) using iTaq universal SYBR green supermix (Bio-Rad) with primers listed in Supplementary Table 4. mRNA expression was quantified as described previously (18).

    Isolation:

    Article Title: Alkylating agents activate an SLFN11-dependent vulnerability that confers PARP-1 inhibitor sensitivity in kidney cancer.
    Article Snippet: Immunofluorescent analysis was carried out with a Zeiss Observer Z1 fluorescence microscope. .. Quantitative reverse transcription-polymerase chain reaction (qRT-PCR) assay Total RNA was isolated using TRIzol reagent (ThermoFisher) and cleaned with RNase-free DNase I (ThermoFisher). cDNA was synthesized using the iScript cDNA synthesis kit (Bio-Rad). .. Realtime PCR was performed by a CFX-96 real-time system (Bio-Rad) using iTaq universal SYBR green supermix (Bio-Rad) with primers listed in Supplementary Table 4. mRNA expression was quantified as described previously (18).

    Synthesized:

    Article Title: Alkylating agents activate an SLFN11-dependent vulnerability that confers PARP-1 inhibitor sensitivity in kidney cancer.
    Article Snippet: Immunofluorescent analysis was carried out with a Zeiss Observer Z1 fluorescence microscope. .. Quantitative reverse transcription-polymerase chain reaction (qRT-PCR) assay Total RNA was isolated using TRIzol reagent (ThermoFisher) and cleaned with RNase-free DNase I (ThermoFisher). cDNA was synthesized using the iScript cDNA synthesis kit (Bio-Rad). .. Realtime PCR was performed by a CFX-96 real-time system (Bio-Rad) using iTaq universal SYBR green supermix (Bio-Rad) with primers listed in Supplementary Table 4. mRNA expression was quantified as described previously (18).

    cDNA Synthesis:

    Article Title: Alkylating agents activate an SLFN11-dependent vulnerability that confers PARP-1 inhibitor sensitivity in kidney cancer.
    Article Snippet: Immunofluorescent analysis was carried out with a Zeiss Observer Z1 fluorescence microscope. .. Quantitative reverse transcription-polymerase chain reaction (qRT-PCR) assay Total RNA was isolated using TRIzol reagent (ThermoFisher) and cleaned with RNase-free DNase I (ThermoFisher). cDNA was synthesized using the iScript cDNA synthesis kit (Bio-Rad). .. Realtime PCR was performed by a CFX-96 real-time system (Bio-Rad) using iTaq universal SYBR green supermix (Bio-Rad) with primers listed in Supplementary Table 4. mRNA expression was quantified as described previously (18).



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    Ligation‐independent cloning scheme. ( A ) Schematic diagram of the gene coding for the fusion construct on the vector suBLA. ( B ) LIC site with <t>BseR1</t> recognition site indicated in bold and cut site indicated with triangles. Proximal cytosine is indicated in red. ( C ) Cleavage of vector by BseR1 disrupts stop codon. ( D ) Treatment of cleaved vector with T4 polymerase in presence of dCTP degrades 3’ ends at the cut site back to proximal cytosines, generating large overhangs. ( E ) PCR product of insert with LIC handles. Proximal guanine is shown in red. ( F ) Treatment of PCR product with T4 polymerase in presence of dGTP degrades 3’ ends back to proximal guanine, generating overhangs complementary to those on vector. ( G ) Insert annealed into vector. Successful insertion converts the stop codon at the BseR1 cut site to TCT, coding for serine.
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    Image Search Results


    Ligation‐independent cloning scheme. ( A ) Schematic diagram of the gene coding for the fusion construct on the vector suBLA. ( B ) LIC site with BseR1 recognition site indicated in bold and cut site indicated with triangles. Proximal cytosine is indicated in red. ( C ) Cleavage of vector by BseR1 disrupts stop codon. ( D ) Treatment of cleaved vector with T4 polymerase in presence of dCTP degrades 3’ ends at the cut site back to proximal cytosines, generating large overhangs. ( E ) PCR product of insert with LIC handles. Proximal guanine is shown in red. ( F ) Treatment of PCR product with T4 polymerase in presence of dGTP degrades 3’ ends back to proximal guanine, generating overhangs complementary to those on vector. ( G ) Insert annealed into vector. Successful insertion converts the stop codon at the BseR1 cut site to TCT, coding for serine.

    Journal: Current Protocols

    Article Title: A Rapid Method to Rank Proteins of Widely Varying Stability: Application to Paramyxovirus Foot Domains

    doi: 10.1002/cpz1.70479

    Figure Lengend Snippet: Ligation‐independent cloning scheme. ( A ) Schematic diagram of the gene coding for the fusion construct on the vector suBLA. ( B ) LIC site with BseR1 recognition site indicated in bold and cut site indicated with triangles. Proximal cytosine is indicated in red. ( C ) Cleavage of vector by BseR1 disrupts stop codon. ( D ) Treatment of cleaved vector with T4 polymerase in presence of dCTP degrades 3’ ends at the cut site back to proximal cytosines, generating large overhangs. ( E ) PCR product of insert with LIC handles. Proximal guanine is shown in red. ( F ) Treatment of PCR product with T4 polymerase in presence of dGTP degrades 3’ ends back to proximal guanine, generating overhangs complementary to those on vector. ( G ) Insert annealed into vector. Successful insertion converts the stop codon at the BseR1 cut site to TCT, coding for serine.

    Article Snippet: suBLA vector miniprep (see Strategic Planning) 10× restriction enzyme buffer (e.g., rCutSmart Buffer, New England Biolabs, cat. no. B6004S, supplied with BSER1) H 2 O, RNase‐free BseR1 restriction enzyme (e.g., New England Biolabs, cat. no. R0581S) Alkaline phosphatase, calf or shrimp (e.g., New England Biolabs, cat. nos.

    Techniques: Ligation, Cloning, Construct, Plasmid Preparation

    Troubleshooting Guide for Ligation‐Independent Cloning

    Journal: Current Protocols

    Article Title: A Rapid Method to Rank Proteins of Widely Varying Stability: Application to Paramyxovirus Foot Domains

    doi: 10.1002/cpz1.70479

    Figure Lengend Snippet: Troubleshooting Guide for Ligation‐Independent Cloning

    Article Snippet: suBLA vector miniprep (see Strategic Planning) 10× restriction enzyme buffer (e.g., rCutSmart Buffer, New England Biolabs, cat. no. B6004S, supplied with BSER1) H 2 O, RNase‐free BseR1 restriction enzyme (e.g., New England Biolabs, cat. no. R0581S) Alkaline phosphatase, calf or shrimp (e.g., New England Biolabs, cat. nos.

    Techniques: Ligation, Cloning, Plasmid Preparation, Control